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UV-Vis spectroscopy is an analytical technique for measuring light absorbance by substances in solution, crucial for chemical analysis. The Beer-Lambert Law relates absorbance to concentration and path length, enabling precise quantification of solutes. Understanding these principles is key for applications in various scientific fields, from biochemistry to material science.
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UV-Vis spectroscopy is a widely used analytical method for determining the absorbance of light by chemical substances in a solution
Cuvettes
A transparent cuvette, typically made of quartz, is used to hold the sample solution in a UV-Vis spectroscopy experiment
Spectrophotometer
A spectrophotometer is used to direct light through the sample and measure the intensity of transmitted light in a UV-Vis spectroscopy experiment
The resulting data are plotted to produce an absorbance spectrum, indicating how much light is absorbed at each wavelength
The Beer-Lambert law is a linear relationship that correlates the absorbance of a sample to its concentration and the path length of the cuvette
Molar absorptivity is the proportionality constant in the Beer-Lambert law and is intrinsic to each substance at a given wavelength
The absorbance spectrum is a graphical representation of the Beer-Lambert law and plots absorbance against wavelength
The absorbance spectrum is essential for identifying the electronic transitions of molecules and understanding their electronic structure
Sample absorbance can be quantified by comparing the intensity of light before and after passing through the sample using the Beer-Lambert law
The concentration of a substance can be calculated using the Beer-Lambert law if the molar absorptivity and path length are known
Deviations from linearity can occur in the Beer-Lambert law due to high solute concentrations, intermolecular interactions, or light scattering
00
______-______ spectroscopy is utilized to measure how much light a chemical substance in solution absorbs.
Ultraviolet-visible
UV-Vis
01
In a ______ experiment, light absorption is measured from ______ to ______ nanometers using a spectrophotometer.
UV-Vis spectroscopy
200
800
02
Absorbance indication in UV-Vis spectroscopy
Decrease in light intensity after sample interaction signifies light absorption by the sample.
