Paper chromatography is an analytical method used to separate mixture components based on solubility. It involves a stationary phase of water-impregnated paper and a mobile solvent phase. Components are separated by their differing affinities for these phases, and their migration rates are quantified by retention factors (Rf values). This technique is useful for identifying substances in a mixture and has applications in various fields, including environmental testing and drug analysis.
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The solvent that carries the components of the mixture through the stationary phase
The paper impregnated with a polar substance that interacts with the analytes
Dimensionless numbers used to quantify the rate of migration of each component in the mixture
A line drawn on the chromatography paper to mark the starting point for the sample
The liquid used to carry the components of the mixture through the stationary phase
The process of examining the chromatogram to identify and compare the components of the mixture
Cost-effective, simple, and requires a small amount of sample
Separation of plant pigments, purification of substances, drug analysis, and environmental testing
Introduces students to the fundamental concepts of chromatography
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Mobile phase in paper chromatography
Solvent that moves through stationary phase, carrying mixture components.
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Stationary phase in paper chromatography
Paper impregnated with polar substance, typically water, for partitioning.
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Retention factor (Rf value) significance
Ratio reflecting component's affinity for stationary vs mobile phase; used for identification.
