Lambda Phage, a temperate bacteriophage infecting E. coli, is pivotal in genetic research, offering insights into lysogenic and lytic cycles. Its structural features, including a polyhedral head and tail, enable DNA injection into host cells. As a genetic engineering tool, Lambda Phage facilitates gene cloning and the study of bacterial resistance, notably against mechanisms like CRISPR-Cas. Its cos sequence is crucial for DNA packaging and integration, making it a model organism in biotechnology and therapeutic research.
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The phage's DNA integrates into the bacterial chromosome and is replicated without causing harm
Molecular Interactions and Regulation
The decision between the lysogenic and lytic pathways is regulated by a complex network of molecular interactions and is influenced by environmental factors and the physiological state of the host cell
The phage hijacks the host's cellular machinery to produce new phage particles, leading to the lysis and death of the bacterial cell
Lambda Phage possesses a distinctive structure comprising a polyhedral head, a tail structure, and a baseplate that assists in anchoring the phage to the bacterial cell surface
Lambda Phage is employed as a cloning vector for precise genetic manipulations and the construction of genomic libraries
Lambda Phage's ability to carry foreign DNA fragments into E. coli cells makes it a powerful tool for cloning large genes or operons
The phage's ability to package and deliver high molecular weight DNA with high efficiency is due to its 'headful' packaging mechanism
The study of interactions between Lambda Phage and bacterial defense systems provides insights into how bacteria can resist phage infections
Lambda Phage is a valuable model for studying the CRISPR-Cas adaptive immune system in bacteria, which has implications for genome editing and understanding bacterial immunity
The study of Lambda Phage enriches our understanding of microbiology, genetic exchange mechanisms, and bacterial resistance strategies
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______ Phage is a temperate bacteriophage known for infecting ______ bacteria and can undergo a lysogenic or lytic life cycle.
Lambda
Escherichia coli (E. coli)
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Lambda Phage Genome Size and Composition
Approx. 48,502 base pairs of linear double-stranded DNA with cos sites.
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Lambda Phage Gene Functionality
Encodes ~60 genes for lytic/lysogenic cycles and phage assembly/function.
