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DNA Structure and Function

DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Each cell's DNA contains the genetic instructions for development and function. The molecule's structure is a double helix, with two strands made of sugar-phosphate backbones and nitrogenous bases. These bases pair specifically (A with T, C with G) to form the genetic code. Understanding DNA's structure, from the double helix to base pairing, is crucial for grasping its role in replication and protein synthesis, pivotal in genetics and medicine.

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1

DNA's primary components

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Nucleotides: composed of a nitrogenous base (A, T, C, G), deoxyribose sugar, and phosphate group.

2

DNA's structural shape

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Double helix: resembles a twisted ladder with sugar-phosphate backbones and base pairs as rungs.

3

Function of DNA base pairs

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Base pairs (A-T, C-G) form the rungs of the helix and carry genetic information through sequences.

4

In DNA, the nitrogenous bases adenine pairs with ______, and cytosine pairs with guanine, a process crucial for DNA's double helix structure and precise duplication.

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thymine

5

DNA molecule shape

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Double helix, two strands twisted around each other.

6

DNA strand directionality

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Each strand has 5' to 3' orientation, referencing carbon numbers in sugar.

7

Role of DNA structure in replication and transcription

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Enables enzyme recognition and interaction for copying and reading genetic info.

8

______ is crucial for maintaining genetic information from one cell generation to the next.

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DNA replication

9

During DNA replication, enzymes known as ______ facilitate the addition of nucleotides based on complementary base pairing.

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DNA polymerases

10

Codon function in protein synthesis

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Each DNA codon specifies a single amino acid in a protein sequence.

11

mRNA's role in protein synthesis

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mRNA carries the genetic code from DNA to the ribosome for translation.

12

Translation location in cells

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Ribosomes are the cellular sites where mRNA is translated into proteins.

13

The Nobel Prize in Physiology or Medicine was awarded to Watson, Crick, and ______ in ______; however, ______ had already died and could not receive the prize.

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Maurice Wilkins 1962 Rosalind Franklin

14

DNA's molecular structure

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Double helix with antiparallel strands and complementary base pairs.

15

DNA's role in heredity

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Encodes genetic instructions passed from parents to offspring.

16

DNA's function in protein synthesis

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Template for RNA synthesis; RNA translates to form proteins.

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The Fundamental Structure of DNA

Deoxyribonucleic acid (DNA) is the molecule that carries the genetic instructions used in the growth, development, functioning, and reproduction of all known living organisms and many viruses. DNA is a long polymer made from repeating units called nucleotides, each of which is made up of a nitrogenous base (adenine [A], thymine [T], cytosine [C], or guanine [G]), a sugar molecule (deoxyribose), and a phosphate group. The structure of DNA is a double helix, which resembles a twisted ladder. The sugar and phosphate constitute the sides of the ladder, while the base pairs form the rungs.
Detailed 3D rendering of a DNA double helix with blue sugar-phosphate backbones and red, yellow, green, orange base pairs against a soft gradient background.

Nucleotide Composition and Base Pairing

Nucleotides, the building blocks of DNA, consist of three components: a phosphate group, a deoxyribose sugar, and one of four nitrogenous bases. The sugar and phosphate groups of adjacent nucleotides link to form the backbone of the DNA strands, with the bases extending inward. Nitrogenous bases are of two types: purines (adenine and guanine), which have a double-ring structure, and pyrimidines (cytosine and thymine), which have a single-ring structure. Base pairing occurs between the nitrogenous bases of the two strands, with adenine pairing with thymine and cytosine pairing with guanine, held together by hydrogen bonds. This complementary base pairing is essential for the double helix structure and the accurate replication of DNA.

The Double Helix and Antiparallel Strands

The DNA molecule is characterized by its iconic double helix shape, formed by two antiparallel strands twisted around each other. Each strand has a directionality, with one end designated as 5' (five prime) and the other as 3' (three prime), referring to the numbering of carbons in the sugar molecule. The antiparallel orientation is critical for the biological processes of DNA replication and transcription, as it allows the enzymes that mediate these processes to recognize and interact with the DNA molecule properly.

DNA Replication and the Role of Complementary Base Pairing

DNA replication is a vital process that ensures genetic continuity from one generation of cells to the next. During replication, the two strands of the DNA double helix separate, and each serves as a template for the synthesis of a new complementary strand. Enzymes called DNA polymerases add nucleotides to the growing strand by matching the bases with their complements (A with T and C with G). This complementary base pairing ensures that each new DNA molecule is an exact replica of the original, preserving the genetic information.

DNA's Function in Protein Synthesis

DNA plays a crucial role in protein synthesis, which is the process by which cells build proteins. The sequence of nucleotides in the DNA specifies the sequence of amino acids in a protein, with each triplet of bases, known as a codon, corresponding to a specific amino acid. This genetic code is nearly universal among organisms. During protein synthesis, the information encoded in DNA is transcribed into messenger RNA (mRNA), which then travels to the ribosome where it is translated into a polypeptide chain, forming a protein.

Historical Discovery of DNA's Structure

The elucidation of DNA's double helix structure is a milestone in the history of science. In 1953, James Watson and Francis Crick proposed the double helix model of DNA, drawing on the experimental data of other scientists, notably Rosalind Franklin, whose X-ray diffraction images were critical in revealing the helical form of DNA. Maurice Wilkins also played a role in the discovery by sharing Franklin's data with Watson and Crick. The Nobel Prize in Physiology or Medicine was awarded to Watson, Crick, and Wilkins in 1962; Franklin had passed away by then and was not eligible for the Nobel Prize, which is not awarded posthumously.

DNA Structure - Key Takeaways

DNA is a complex, double-helical molecule that encodes the genetic instructions for living organisms. Its structure, featuring antiparallel strands linked by complementary base pairs, is fundamental to its function in heredity, replication, and protein synthesis. The discovery of the double helix and the understanding of DNA's role in biology have been instrumental in advancing the fields of genetics, biotechnology, and medicine. A comprehensive grasp of DNA structure and function is essential for students and professionals in the life sciences.