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DNA replication is crucial for genetic fidelity during cell division, with stress leading to potential diseases like cancer. PCR amplifies DNA for research and diagnostics. Both processes have broad implications in biology and technology, influencing medical treatments and data storage solutions.
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Replication stress can be triggered by various factors such as ribonucleotide incorporation, DNA secondary structures, and collisions between replication and transcription machineries
Replication stress can result in genomic instability, which is a hallmark of many diseases, including cancer
Understanding and mitigating replication stress is crucial for maintaining cellular health and preventing disease progression
PCR involves cycles of heating, cooling, and DNA synthesis to selectively amplify specific DNA sequences
PCR has become an indispensable tool for genetic research, medical diagnostics, and forensic science due to its ability to rapidly and accurately amplify DNA
The development of PCR has revolutionized the field of molecular biology and has led to advancements in DNA amplification techniques
DNA replication involves the unwinding of the DNA double helix, synthesis of new DNA strands, and reconstitution of chromatin structure to ensure the faithful transfer of genetic information
Accurate DNA replication is vital for cell division, allowing each new cell to inherit a complete set of genetic instructions and preventing potential mutations
The correct distribution of chromosomes during cell division relies on the precise replication of DNA, making it fundamental to the continuity of life and the prevention of genetic disorders
Epigenetics involves modifications that affect gene expression without altering the DNA sequence itself
Stalled replication forks can lead to alterations in chromatin structure, potentially influencing gene activity and passing on epigenetic changes to daughter cells
Investigating the epigenetic effects of replication stress is crucial for understanding how cells can develop new characteristics and how diseases such as cancer may arise and evolve due to changes in gene regulation