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Exploring the intricacies of DNA replication in eukaryotes, this overview highlights the roles of DDK, Sld3, Sld2, and Dpb11 in replication initiation. It delves into the formation of the replisome, the mechanics of the replication fork, strand synthesis, and the maturation of Okazaki fragments. The text also details the functions of the three main replicative DNA polymerases, Pol α, Pol δ, and Pol ε, in maintaining the fidelity and efficiency of DNA replication.
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DDK promotes the attachment of Cdc45 to replication origins by phosphorylating the Mcm complex
Role of Sld3 and Sld2
Sld3 and Sld2 interact with Dpb11, which is necessary for CDK-mediated activation of DNA replication
Role of Dpb11
Dpb11 interacts with DNA Polymerase ε and the GINS complex to regulate DNA replication
The replisome coordinates DNA synthesis and includes factors such as DNA polymerases, Claspin, And1, and RFC clamp loader
The replication fork separates parental DNA into single strands for replication
DNA polymerases synthesize the leading and lagging strands in opposite directions
The maturation of Okazaki fragments involves the removal of RNA primers, gap filling, and ligation to complete the lagging strand
Polymerase α initiates DNA synthesis by creating a short RNA-DNA primer
Polymerase switching is necessary for the continuation of DNA synthesis, with Pol ε extending the leading strand and Pol δ synthesizing the Okazaki fragments on the lagging strand
Replicative DNA polymerases work with other factors to ensure the accurate and complete replication of the eukaryotic genome