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DNA replication is crucial for genetic inheritance and cell division, involving enzymes like helicase and DNA polymerase. This process is semi-conservative, creating identical DNA molecules for new cells. Laboratory techniques, such as PCR, replicate DNA for research and diagnostics, impacting our understanding of evolution and the origin of life. Mutations during replication contribute to genetic diversity and evolution, while studies on RNA suggest insights into life's beginnings.
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DNA is composed of two antiparallel strands, each made up of nucleotide bases paired together by hydrogen bonds
Meselson-Stahl Experiment
The Meselson-Stahl experiment in 1958 demonstrated that DNA replication is a semi-conservative process
Origins of Replication
DNA replication begins at specific sites called origins of replication, where the DNA double helix is unwound to form a replication fork
DNA replication involves the coordinated action of proteins and enzymes, including helicase, primase, DNA polymerase, and ligase, to synthesize new DNA strands using the original strand as a template
Techniques such as PCR, LCR, and TMA use the principles of DNA replication to amplify and study specific DNA sequences in the laboratory
PCR uses thermal cycling to denature, anneal, and extend DNA strands, allowing for the exponential amplification of target sequences
LCR is a technique that utilizes DNA ligase to amplify specific DNA sequences
TMA is a technique that uses RNA polymerase to amplify specific RNA sequences
DNA replication is a driving force of evolution, introducing mutations that contribute to genetic diversity and adaptation
The study of DNA replication provides insights into the origin of life, including the potential role of RNA molecules as early genetic material and catalysts in prebiotic environments