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Exploring the termination of DNA replication in E. coli, this overview highlights the role of Ter sites and the Tus protein in halting replication forks. It delves into the catenation and decatenation of E. coli chromosomes by Topoisomerase IV, ensuring proper cell division. Additionally, it touches on alternative prokaryotic DNA replication models, including rolling circle replication in bacterial conjugation and D-loop replication in organellar DNA, showcasing the diversity of replication strategies in prokaryotes.
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Ter sites and Tus protein work together to halt the replication fork and prevent over-replication in E. coli
Topoisomerase IV enzyme
Topoisomerase IV resolves catenanes by introducing breaks and re-ligating DNA strands in E. coli
Prokaryotes have diverse replication methods, such as rolling circle and D-loop replication, adapted to their environments and biological functions
The relaxase enzyme initiates rolling circle replication by nicking the oriT in bacterial conjugation
The F-plasmid system, consisting of TraI, TraY, TraM, and IHF, forms a relaxosome complex for rolling circle replication in bacterial conjugation
Rolling circle replication in bacterial conjugation allows for the transfer of genetic material and contributes to genetic diversity among bacterial populations
D-loop replication in organellar DNA involves the formation of a displacement loop, which serves as a primer for DNA synthesis
D-loop replication in organellar DNA is regulated by enzymes and specific DNA sequences to ensure accurate and efficient replication in essential cellular organelles
D-loop replication is critical for maintaining the genomes of mitochondria and chloroplasts in eukaryotic cells
00
Ter sites in the E. coli genome act as ______ to replication forks, stopping the unwinding of the double helix.
unidirectional barriers
01
The ______ stops the replication fork to prevent it from going past the replication terminus in E. coli.
Tus-Ter complex
02
The termination mechanism in E. coli is vital for avoiding ______ and ensuring correct distribution of DNA to daughter cells.
over-replication
